All functions

Cq.data.df()

This Function will give the data.cq samples for one Cq type in a dataframe

  • helper function.

Cq.data.mean()

This Function will summarize the data.cq samples for one Cq type!

  • helper function.

calc.Cq()

calculates Cq values

calc.Cq.qpcR()

calculates Cq values

combineSubsamples()

This Function will combine subsamples, so that they can be used as one sample.

delta.Cq.data()

This Function will give the delta Cq values. Always: offtarget Cq - target Cq

  • helper function.

linSqrtTransform()

This Function will linearise the Delta Cq data with a shifted square root transformation.

  • helper function.

make.Cq.data()

This Function will create the main Data object for all analysis. Used after an input is created with read.CqTable and all optional calculation data validation is complete.

plot.Cq()

plots Cq values with the both genotypes on x and y axis

plot.Cq.efficiency()

plots Cq values of each genotype againgst the pseudo log concentration.

plot.curve()

plot fluorescence curves

plot.delta.Cq.box()

plots delta Cq values as box-plots

plot.delta.Cq.differences()

plots delta Cq values against the square root of the concentration

plot.delta.Cq.overview()

plots delta Cq values against the concentration

reTransform()

This Function will retransform a value / list etc to normal percentage / proportion

read.cqTable()

Load cq table

read.fluorescenceTable()

Load fluorescence table

regression.delta.Cq()

This function will build a regression model on the delta Cq values. Plot and cross validation is included.

table.Cq()

shows Cq values from a sample and the mean Delta Cq

voges_dixon()

Helper function for outlier detection.

voges_grubbs()

Helper function for outlier detection.