WRAPPER for qpcR Package 'qpcR: Modelling and Analysis of Real-Time PCR Data, Andrej-Nikolai Spiess, 2018'
calc.Cq.qpcR(method = "TP", fct = l4, cq.new = "calc.qpcR.Cq")The method used to calculate the Cq value. "TP / cpD1" for Turning Point (first derivative) . "SD / cpD2" for first exponential incline (second derivative). "cpE", "cpR", "cpT", "Cy0", "cpCQ", "cpMR" (see qpcR package)
will be used by qpcR as fitting function. l4, l5, b4
The Column name of the new Cq values.
does not return! adds Cq value to input.cq data frame
This function will calculate Cq values for the data frame input.cq You need to have input.cq and input.raw from "read.fluorescenceTable" / "read.cqTable" or build them manually.
Please note, that it is faster to use qpcR directly!